|BioTech FYI Center - Resources
Transformation in Tobacco Leaf Disks -
provides a tutorial protocol for the transformation of eukaryotic plant cells
via infection with Agrobacterium.
includes micropropagation (through somatic cell embryogenesis), transformation
by DNA coated microprojectiles, Agrobacterium-mediated transformation,
and preparing liquid culture media.
Transformation Procedures -
includes wheat tissue culture recipes, sterile dissection, culture, and
transformation of wheat immature embryos, biolistic transformation, molecular
characterization of transgenic plants, etc.
- How to Collect
Voucher Specimens -
explains how to collect, preserve, and label plants and pollen for use as an
indicator of where insects are likely to settle or forage. Although text-heavy,
it provides a respectable overview on how to dry and press plants for
Vegetative Propagation -
explains some general procedures for the artificial stimulation of vegetative
propogation in many types of plants. Methods covered inlcude stem cutting, leaf
cutting, simple layering, air layering, and tissue culture.
Plants From Embryonic Plant Tissue -
a very simple teaching laboratory, targeting first-year undergraduate and
high-school biology students.
- Plant Tissue
a concise overview of plant micropropagation for the novice. The document
focuses on encouraging plant enthusiasts with little biological background to
carry out their own experiments in tissue culture to reproduce their favorite
plants for enjoyment or business.
- Dendrome Laboratory Protocols -
a good basic set of tools for molecular genome analysis with a special emphasis
on application to trees, and organisms related to forest health.
- How To Diagnose
Black Walnut Damage -
a diagnostic reference for the various ailments that might be afflicting
your favorite black walnut tree. Each of the most common assaults on black
walnut health is then linked to detailed descriptions of the problem with
recommendations for alleviating it.