|BioTech FYI Center - Resources
General Microbiology Protocols
- Technical Tips Online -
a forum supported by Elsevier Science which allows scientists in multiple
disciplines to communicate technical information to the rest of the scientific
community. The source of each protocol or tip in this rather large database is
very well documented.
- Lab Methods -
techniques include smear preparation and Gram-staining procedure. Test
include catalase, citrate, coagulase, and indole test.
Protocols for Pathogens
Diseases Laboratory Notebook -
contains information about how to maintain an infectious disease lab
notebook. Offers methods and procedures, including instructions for Gram
- Diagnosis of
Helicobacter pylori -
an informative site that contains a simple explanation of several techniques
for testing for H. pylori, the bacterium that causes stomach ulcers.
Assorted Specific Protocols
Protocols for Cyanobacteria -
includes a pigment calculation program, S-State simulation program,
protocol for assaying phycobiliproteins in Fremyella diplosiphon,
and cryopreservation techniques.
- Bacterial Transformation by Electroporation -
a basic protocol for the transformation of E. coli cells by
electroporation. Included within the protocol are basic instructions on growing
and preserving electrocompetent cell aliquots.
- Golden Lab
offers several well-written molecular biology protocols related to
Synechococcus. Specifically, the page describes transposon mutagenesis,
conjugal plasmid transfer from E. coli, preserving cyanobacteria strains
via freezing, and RNA extraction using hot phenol.
- Gregarine Protocol
contains some procedures common to the study of gregarines. Included are
methods for staining, mounting, and fixing these protozoans.