Colony PCR -
provides a basic overview of the use of colony PCR in detecting recombinative
events, both homologous and non-homologous from suspended S. pombe
colonies.
Fission Yeast
Transformation Procedures -
includes three methods for transforming S. pombe with recombinant DNA:
electroporation, lithium acetate treatment, and protoplast fusion.
Gel-Purified,
Agarased YACs for Yeast Transformation -
a protocol for purifying yeast artificial chromosomes by gel electrophoresis
and retrieving the resultant products. The products are suitable for use in
transformation experiments.
Herskowitz Lab Protocols -
provides a wide variety of Saccharomyces cerevisiae protocols and
supporting information. Covers topics including transformation, PCR,
mutagenesis, and biochemical assays.
Microsome
Preparation -
a simple method for the isolation and preservation of microsomes from
S. cerevisiae.
Working With
Fission Yeast -
contains a simple and effective protocol for the preparation of S.
pombe cells for flow cytometry.
Fungal
Genetics Stock Center Methods Page -
offers advice, and a number of protocols dealing with microbiological problems
in fungal genetics. The protocols center around cross conditions, culture
media, and handling technique for Neurospora and Aspergillus
species. The methods are presented in essay format, so speed of reading is
sacrificed in favor of a detailed background for each set of protocols.
A Short 'Shroom
Primer -
a brief overview of many of the most common methods of edible mushroom
identification. This guide does not provide information about identification of
specific fungal species, but rather describes a group of tools which contribute
to the accuracy of mushroom identification. The page also discusses the
environmental and legal impacts of mushroom collection.
