|BioTech FYI Center - Resources
Lab Animal Care
Jackson Laboratory Induced Mutant Resource Genotyping Protocols -
basic procedures for mouse-related molecular biology are posted, such as mouse
DNA isolation from tail biopsies, and blood.
and Application Guides -
includes techniques for plasmid purification and extraction, isolation of
genomic DNA from blood or tissue (including mouse tails), the use of
electrophoresis products, and the use of protein and peptide HPLC columns.
- Protocols for Big
Blue Experiments -
contains some DNA and tissue manipulation protocols for use with the Big Blue
system for mouse transgenics. Techniques include: tissue isolation from bone
marrow and colon, DNA purification and sequencing through bacteriophage lambda
vector, and selection for marker genes.
Lab Protocols -
procedures (some of which are illustrated) for embryonic dissection, mounting,
and in situ hybridization. In addition, the page recommends a PCR genotyping
protocol, and a method for immunostaining of whole mounted tissue. A nice set
of tools for mouse genetics.
- Schimenti Lab
Technical Protocols (The Joy of Cloning) -
listed are basic techniques for DNA isolation (including agarose and acrylamide
gel purification) and extraction from various tissues (including mouse tail and
brain preparations). The manual also describes such techniques as spin column
chromatography, Southern blotting, colony screening, and cell culture
- Comprehensive Protocol Collection -
an excellent methods page dedicated to techniques relating to
C. elegans, both on a macro- and microscopic level.
- Jorgensen Lab Protocol Database -
a comprehensive molecular biology resource with a large variety of methods and
protocols relating to nucleic acids and proteins, with a focus on applications
relating to nematodes. Also provided are techniques for PCR, Northern &
Southern blotting, bacterial and yeast transformation, light microscopy,
electron microscopy, microinjection, and high-yield DNA preps.
- Protocols -
a protocol for Tc1 mediated target selected gene inactivation via reverse
genetics is presented for use in nematodes. This technique is prefaced by an
explanation of reverse genetics and its applied use in the study of the
nematode genome. Also described is a simple protocol for transposon insertion
Other Zoological Methods
- National Zoological Park Genetics Technical Corner -
technical details of laboratory protocols and genetic analyses that have been
used or developed in the Molecular Genetics Laboratory at the National Zoo.
Specifically, it includes bead and nylon versions of their highly detailed
microsatellite cloning protocol (available in MS Word, RTF, and .sit and .zip
- Methods for
Preserving Amphibians and Reptiles for Scientific Study -
a simple overview of how to preserve herpetological specimens. It covers
euthanizing, injection and slitting, fixing, labeling, and storage.