How can I limit my search to a "subset" of ...

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How can I limit my search to a "subset" of database sequences?

You can use the "Limit by Entrez Search" option found on most Standard BLASTR search pages to run an Entrez search and have your query sequence compared to the resutls of this search. For example, if you wanted to limit you search to all phosphorylase sequences from mouse you could enter the following valid Entrez search strategy in the Limit by Entrez field of the BLAST search page: phosphorylase AND "Mus musculus"[Organism]

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Other Frequently Asked Questions

• How can I write a program to submit jobs to NCBI's BLAST servers?
• Q: Which BLAST program should I use?
• I have heard that I will be penalized if I send a large number of sequences to the servers?
• What are other Molecular Biology Resources?
• Causes for "No significant similarity found"
• Can I use BLAST to compare to two or more sequences in a multiple sequence alignment?
• Is it possible to search for a motif or pattern with BLAST?
• What is the Expect (E) value?
• Why do I get the message "ERROR: Blast: No valid letters to be indexed"?
• How do I perform a similarity search with a short peptide/nucleotide sequence?
• More...