Date: 09-Apr-2013
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Biochemist-Protein Chemist
· Molecular cloning and site-directed mutagenesis
· Protein Purification using various chromatographic techniques
· Analytical characterization of purified protein
· Characterize protein-protein interactions
· Characterize protein structure, stability, and solution properties using various biochemical and biophysical approaches
· BS with 2-8 years or MS with 0-6 years post degree experience in an academic or industrial setting working with proteins.
· Hands-on experience with cloning, purification, and characterization of proteins
· Capable of independently designing experiments, generating data, interpreting results, and documenting work
· Experience in purification of non-tagged, low expression, or otherwise difficult to purify proteins
· Molecular biology experience to optimize gene expression (signal peptide selection, codon optimization, mRNA structure) and use of standard DNA software (Vector NTI and Sequencer)
· Preformulation stability analysis and excipient selection
· In silico structural analysis to aid mutagenesis selection
· Experience in creating/optimizing new analytical separation methods (SEC, RP,
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BioTech FYI Center
http://biotech.fyicenter.com/
