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please visit http://biotech.fyicenter.com/faq/
(Continued from previous question...)
How do I get the reverse-complement of a sequence using the subseq
method?
One way is to pass the location to subseq
in the form of a Bio::LocationI object. This object holds strand information as
well as coordinates.
use Bio::Location::Simple;
my $location = Bio::Location::Simple->new(-start => $start,
-end => $end,
-strand => "-1");
# assume we already have a sequence object
my $rev_comp_substr = $seq_obj->subseq($location);
(Continued on next question...)