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Bioinformatics Glossary
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(Continued from previous part...)
Yeast 2-hybrid system
A yeast-based method used to simultaneously identify, and clone the
gene for, proteins interacting with a known protein. The basis of this
method is a "transcriptional reporter assay" (see definition) in which
reporter gene expression is dependent on two domains. The first domain
is linked to the known protein. The second domain is genetically linked
to a library. If the library is screened against the known protein the
two domains will interact only if a protein from the library binds the
known protein, resulting in transcription activation of the reporter gene,
and a blue color. The "blue yeast clone" will contain the gene encoding
the newly identified protein.
(Continued on next part...)
Part:
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
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