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Nick translation
Refers to the in vitro procedure used to radioactively label DNA uniformly to a high specific activity. Nicks are introduced into the unlabelled DNA by an endonuclease, thus creating 3' hydroxyl termini. E. coli DNA polymerase then adds radioactive residues to the 3' hydroxy terminus of the nick; nucleotides are removed from the 5' side. This procedure leads to an identical DNA molecule with the nick located further along the duplex.
Nucleotide
Monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose, and a phosphoric acid group. The nucleotides of DNA are deoxyadenylic acid, thymidylic acid, deoxyguanilic acid, and deoxycytidylic acid. Those of RNA are adenylic acid, uridylic acid, guanylic acid and cytidylic acid.
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