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BAC
Stands for Bacterial Artificial Chromosome, a cloning and sequencing vector derived form a bacterial chromosome into which a 100,000 bp fragment or more can be inserted.
BioChip
Miniaturised substrates onto which a large number of biomolecules are attached with high density and in a defined microarray.
Bioinformatics
Science dealing with the classification, storage, retrieval and analysis of genomic and proteomic information; molecular modelling.
Biosensor
Device in which recognition systems of biological chemicals are coupled to microelectronics to allow the low-level detection of substances such as proteins etc. in body fluids or pollutants in water.
Biotinylation
DNA probes are labelled with biotin. Biotinylated triphosphates are incorporated into the molecule by enzyme-dependent labelling reactions such as e.g. nick translation or random primed labelling. Afterwards, the probe is hybridised to the target. The location of biotin is visualised by complexing it with a streptavidin molecule that is attached to a colour-generating agent.
BLAST
Heuristic homology search algorithm
Bootstrap
Recalculation of results with randomly rearranged datasets to exclude the possibility of artefacts in data clustering.
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