Biotech > Glossary

Microarray Glossary

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Radioimmunoassay
= RIA: very sensitive diagnostic test using radioactively labeled specific antibodies or antigens to detect trace amounts of substances.


Reader
Synonym for microarray scanner; after fluorescent labelling and hybridisation, the reader scans the microarrays into a computer for subsequent analysis.


Regulatory sequence
A DNA sequence involved in regulating the expression of structural genes in the common operon. These stretches of DNA might be attenuators, operators, or promoters.


RT-PCR
Reverse Transcriptase Polymerase Chain Reaction: The mRNA is first reverse-transcribed into cDNA, and the cDNA is then amplified to measurable levels using PCR. PCR primers for all genes of interest are required.

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