Microarray Glossary

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(Continued from previous part...)

Oligonucleotide chips
Oligonucleotide chips consist of small glass plates with thousands of short 20-mer oligonucleotide probes attached to their surface. The oligos are synthesized directly onto the surface using a combination of semiconductor-based photolithography and light-directed chemical synthesis. Very large numbers can be probed; at present chips contain over 65,000 probes.

Operon
A unit that consists of one of more cistrons which function coordinateley under the control of an operator gene.

ORF
Open Reading Frame; a reading frame refers to a nucleotide sequence that starts with an initiation codon, partitions the subsequent nucleotides into amino-acid-encoding triplets and ends with a termination codon. The interval between the start and stop codons is referred to as the open reading frame.

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